Difference between revisions of "Part:BBa K2929003:Experience"
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===Applications of BBa_K2929003=== | ===Applications of BBa_K2929003=== | ||
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| + | ==iGEM 2019 St Andrews - Part Development== | ||
| + | <html> | ||
| + | <p> The Thermatoga maritima Beta-glucosidase A enzyme expresses well under the T7 promoter in a pET28a, even without IPTG induction, due to a leaky promoter. The protein is mostly soluble based on small scale expression tests and runs at ~55 kDa on an SDS-Page gel.</p> | ||
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| + | <img src="https://2019.igem.org/wiki/images/0/0e/T--St_Andrews--ThermotogaWT.jpg" width="30%"> | ||
| + | <p style="text-align:center;"><font size=-2>Figure 1. Soluble expression for the wild-type protein, the pET28a vector was known to have a leaky promoter so the bands at ~55kDa (expected mass 52kDa) indicate expression, as they are absent in lanes used for mOrange and Antibody expression (lanes 2-10).</p> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:02, 19 October 2019
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Applications of BBa_K2929003
iGEM 2019 St Andrews - Part Development
The Thermatoga maritima Beta-glucosidase A enzyme expresses well under the T7 promoter in a pET28a, even without IPTG induction, due to a leaky promoter. The protein is mostly soluble based on small scale expression tests and runs at ~55 kDa on an SDS-Page gel.
Figure 1. Soluble expression for the wild-type protein, the pET28a vector was known to have a leaky promoter so the bands at ~55kDa (expected mass 52kDa) indicate expression, as they are absent in lanes used for mOrange and Antibody expression (lanes 2-10).
User Reviews
UNIQe5bcd04c7d101c74-partinfo-00000001-QINU UNIQe5bcd04c7d101c74-partinfo-00000002-QINU