Difference between revisions of "Part:BBa K2929002:Experience"
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===Applications of BBa_K2929002=== | ===Applications of BBa_K2929002=== | ||
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| + | ==iGEM 2019 St Andrews - Part Development== | ||
| + | <html> | ||
| + | <p> The Human IgE CH3 Domain expresses well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.</p> | ||
| + | <div align = "center"> | ||
| + | <img src="https://2019.igem.org/wiki/images/3/30/T--St_Andrews--Antibody_13_Expression_Test.jpg | ||
| + | " width="30%"> | ||
| + | <p style="text-align:center;"><font size=-2>Figure 1. Human IgE wild-type expression, showing clear bands corresponding to insoluble recombinant protein for the induced fraction. Soluble recombinant protein expression is not visible on this gel.</p> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 15:01, 19 October 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2929002
iGEM 2019 St Andrews - Part Development
The Human IgE CH3 Domain expresses well at 25°C in SoluBL21 cells in a pEHISTEV vector, with 0.2 mM IPTG induction overnight, but the expressed protein is mostly insoluble based on small-scale expression tests.
Figure 1. Human IgE wild-type expression, showing clear bands corresponding to insoluble recombinant protein for the induced fraction. Soluble recombinant protein expression is not visible on this gel.
User Reviews
UNIQ7d15662f511fb4f8-partinfo-00000001-QINU UNIQ7d15662f511fb4f8-partinfo-00000002-QINU