Difference between revisions of "Part:BBa K2943900"

Revision as of 14:51, 19 October 2019

Improved RBS

This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014).
This sequence is based on the RBS (https://parts.igem.org/Part:BBa_B0032) with several mutations inside and includes also base inserts that increase the Shine Dalgarno(SD) sequence length in order to optimize the expression of the mRFP in the composite part.
The mutations to optimize the SD were made according to the anti-SD sequence on the ribosome and the extra bases which were inserted were according to the the most common optimal distance in E.coli
. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This sequence is part of a composite part (https://parts.igem.org/Part:BBa_K2943902) that aims to improve an existing composite part (https://parts.igem.org/Part:BBa_K608014). <br>
-This sequence is based on the RBS (https://parts.igem.org/Part:BBa_B0032) with several mutations inside and includes also base inserts that increase the Shine Dalgarno sequence length in order to optimize the expression of the mRFP in the composite part.
+This sequence is based on the RBS (https://parts.igem.org/Part:BBa_B0032) with several mutations inside and includes also base inserts that increase the Shine Dalgarno(SD) sequence length in order to optimize the expression of the mRFP in the composite part.   <br>
+The mutations to optimize the SD were made according to the anti-SD sequence on the ribosome and the extra bases which were inserted were according to the the most common optimal distance in <em>E.coli</em><br>.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===