Difference between revisions of "Part:BBa K2963004"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2963004 short</partinfo> | <partinfo>BBa_K2963004 short</partinfo> | ||
| − | This part contains the <i>racE</i> gene encoding racemase from <i>Bacillus sp.</i>. This enzyme mainly catelyses the reation of L- | + | This part contains the <i>racE</i> gene encoding racemase from <i>Bacillus sp.</i>. This enzyme mainly catelyses the reation of L-glutamate to D-glutamate. In our project, we coexpress the <i>racE</i> with <i>capB*CA</i> complex in order to produce poly-γ-glutamic acid with different ratio of L-glutamic acid. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:49, 19 October 2019
racE - a glutamate racemase from Bacillus sp.
This part contains the racE gene encoding racemase from Bacillus sp.. This enzyme mainly catelyses the reation of L-glutamate to D-glutamate. In our project, we coexpress the racE with capB*CA complex in order to produce poly-γ-glutamic acid with different ratio of L-glutamic acid.
Usage and Biology
The racE gene is derived from Bacillus subtilis and it encodes a racemase which can converts L-glutamate to D-glutamate.
Characterization
We used enzyme activity data to prove the usability of this part. We measured the enzymatic activity under different tac promoters.
We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]