Difference between revisions of "Part:BBa K3081112"
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<partinfo>BBa_K3081112 short</partinfo> | <partinfo>BBa_K3081112 short</partinfo> | ||
| − | + | The 20 bp sgRNA targets to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | |
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Revision as of 14:45, 19 October 2019
a 20bp sequence targeting the R4(bottom strand) DnaA box on E.coli oriC
The 20 bp sgRNA targets to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]