Difference between revisions of "Part:BBa K3102043"
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class="c20"><p class="c8"><span class="c7">Results: Comparison of lactate secretion between engineered and non-engineered bacteria </span></p><p class="c8 c22"><span class="c7"></span></p><p class="c9"><span>An important step in our system in the optimization of lactate secretion by </span><span class="c11">E. coli</span><span>. Indeed, lactate constitute the energetic substrate of </span><span class="c11">S. oneidensis</span><span class="c4"> and thus an essential element to the generation of electricity. </span></p><p class="c9"><span class="c4">Lactate detection kit was used as described in “Protocol: Lactate Detection” and the following measurement were recovered:</span></p><p class="c9 c22"><span class="c4"></span></p><a id="t.c0c51514b21a288cf077105e6ffccb79c8a44457"></a><a id="t.0"></a><table class="c18"><tbody><tr class="c3"><td class="c5" colspan="1" rowspan="1"><p class="c2"><span class="c0"> </span></p></td><td class="c5" colspan="1" rowspan="1"><p class="c2"><span class="c0">0h</span></p></td><td class="c5" colspan="1" rowspan="1"><p class="c2"><span class="c0">3h</span></p></td><td class="c5" colspan="1" rowspan="1"><p class="c2"><span class="c0">6h</span></p></td><td class="c5" colspan="1" rowspan="1"><p class="c2"><span class="c0">9h</span></p></td></tr><tr class="c14"><td class="c6 c16" colspan="1" rowspan="1"><p class="c2"><span class="c12">Control </span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">60,235</span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">49,982</span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">24,671</span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">13,136</span></p></td></tr><tr class="c14"><td class="c6 c16" colspan="1" rowspan="1"><p class="c2"><span class="c12">LLOpLdha </span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">69,206</span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">67,925</span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c4">47,740</span></p></td><td class="c6" colspan="1" rowspan="1"><p class="c2"><span class="c15">48,060</span></p></td></tr></tbody></table><p class="c8"><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 514.40px; height: 314.40px;"><img alt="" src="images/image1.png" style="width: 514.40px; height: 314.40px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></span></p><p class="c21"><span class="c4">To have a better view of the efficiency of our modified bacteria, we calculated the following ratios:</span></p><p class="c10"><span class="c4"></span></p><a id="t.fcbaf46168b43ac8baa366cf42d318313bd37c92"></a><a id="t.1"></a><table class="c18"><tbody><tr class="c17"><td class="c1" colspan="1" rowspan="1"><p class="c2"><span class="c0"> </span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c2"><span class="c0">0h</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c2"><span class="c0">3h</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c2"><span class="c0">6h</span></p></td><td class="c1" colspan="1" rowspan="1"><p class="c2"><span class="c0">9h</span></p></td></tr><tr class="c13"><td class="c1" colspan="1" rowspan="1"><p class="c2"><span class="c12">Ratio</span></p></td><td class="c19" colspan="1" rowspan="1"><p class="c2"><span class="c0">1,15</span></p></td><td class="c19" colspan="1" rowspan="1"><p class="c2"><span class="c0">1,36</span></p></td><td class="c19" colspan="1" rowspan="1"><p class="c2"><span class="c0">1,93</span></p></td><td class="c19" colspan="1" rowspan="1"><p class="c2"><span class="c0">3,66</span></p></td></tr></tbody></table><p class="c8"><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 457.60px; height: 271.20px;"><img alt="" src="images/image2.png" style="width: 457.60px; height: 271.20px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></span></p><p class="c21" id="h.gjdgxs"><span class="c4">This data shows clearly the efficiency of the modified organism to secrete more lactate. </span></p></html> | ||
Revision as of 14:21, 19 October 2019
Lactate production (ldhA, lldP, ACS operon)
This "lactate production" composite part is composed of Lactate dehydrogenase (LdhA):BBa_K3102026 and lactate permease (LldP):BBa_K3102027 sequences. This part increases the production and secretion of D-Lactate in the extracellular media. The LDHA enzyme catalyse the interconversion of pyruvate to L-lactate with simultaneous conversion of NADH to NAD+.
This composite part also constains the ACS operon:BBa_K3102028, composed of three genes: actP (Transports acetate), acs (catalyzes the conversion of acetate into acetyl-CoA), and yfcH (epimerase). It allows for the uptake of acetate molecules in order for the bacteria to do correctly its metabolism and survive.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1478
Illegal XhoI site found at 3404 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 489
Illegal AgeI site found at 1614
Illegal AgeI site found at 2077
Illegal AgeI site found at 2515
Illegal AgeI site found at 2674
Illegal AgeI site found at 2686
Illegal AgeI site found at 3833 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 219
Illegal SapI.rc site found at 6698
An important step in our system in the optimization of lactate secretion by E. coli. Indeed, lactate constitute the energetic substrate of S. oneidensis and thus an essential element to the generation of electricity. Consequently, we wanted to compare lactate secretion between engineered and non-engineered bacteria.
How? Method ?
Lactate detection kit was used as described in “Protocol: Lactate Detection” and the following measurement were recovered:
- tableau + Graph
Results: Comparison of lactate secretion between engineered and non-engineered bacteria
An important step in our system in the optimization of lactate secretion by E. coli. Indeed, lactate constitute the energetic substrate of S. oneidensis and thus an essential element to the generation of electricity.
Lactate detection kit was used as described in “Protocol: Lactate Detection” and the following measurement were recovered:
| 0h | 3h | 6h | 9h |
Control | 60,235 | 49,982 | 24,671 | 13,136 |
LLOpLdha | 69,206 | 67,925 | 47,740 | 48,060 |
To have a better view of the efficiency of our modified bacteria, we calculated the following ratios:
| 0h | 3h | 6h | 9h |
Ratio | 1,15 | 1,36 | 1,93 | 3,66 |
This data shows clearly the efficiency of the modified organism to secrete more lactate.