Difference between revisions of "Part:BBa K3182010"
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| − | + | Parseq Beta is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria was combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD<sup>600</sup> was measured. The results can be seen in <b>Figure 1.</b>. | |
[[File:T--Linkoping_Sweden--betaEcoli.png|650px|thumb|center| <b>Figure 1.</b> Shows the antimicrobial activity of Parseq Beta on E.Coli. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.]] | [[File:T--Linkoping_Sweden--betaEcoli.png|650px|thumb|center| <b>Figure 1.</b> Shows the antimicrobial activity of Parseq Beta on E.Coli. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.]] | ||
Revision as of 13:55, 19 October 2019
Parseq-β
Parseq Beta is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria was combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD600 was measured. The results can be seen in Figure 1..
Figure 1. Shows the antimicrobial activity of Parseq Beta on E.Coli. The OD600 value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.
Figure 2. Shows the antimicrobial activity of Parseq Beta on B.Subtilis. The OD600 value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]