Difference between revisions of "Part:BBa K2983018"
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This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites. | This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites. | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 13:49, 19 October 2019
Loop A-alpha
This part is a Loop Type IIS Golden gate adapter that contains a BsaI and a SapI restriction sites.
Usage and Biology
The Loop Type IIS cloning sites (triangles in figure 1) are a combination of BsaI and SapI restriction sites each with different cutting sites that generate well defined overhangs (circles in Figure 1).The sequence of the loop A-Alpha is as fellow : GGTCTCAGGAGAATGTGAAGAGC
Blue triangle: SapI fixation site (GCTCTTC)
Red triangle : BsaI fixation site (GAGACC)
Blue circle : overhang formed by SapI after cutting (GGAG)
Red circle: overhang formed by BasI after cutting (ATG)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal SapI.rc site found at 17