Difference between revisions of "Part:BBa K091122:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence. | + | Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence. The sequencing results showed that base 21 was a T instead of a C, but the resulting codons (TAT and TAC) both code for tyrosine. |
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===Source=== | ===Source=== |
Latest revision as of 16:27, 19 October 2008
LacI_I12 protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence. The sequencing results showed that base 21 was a T instead of a C, but the resulting codons (TAT and TAC) both code for tyrosine.
Source
The basic sequence comes from Ecogene.com