Difference between revisions of "Part:BBa K091122:Design"

 
(Design Notes)
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K091122 short</partinfo>
 
<partinfo>BBa_K091122 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence.
+
Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence. The sequencing results showed that base 21 was a T instead of a C, but the resulting codons (TAT and TAC) both code for tyrosine.
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 16:27, 19 October 2008

LacI_I12 protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because there are many codons that code for the amino acid tyrosine, we chose to use the TAT codon, because it was the most common tyrosine codon found throughout the rest of the LacI wild-type gene sequence. The sequencing results showed that base 21 was a T instead of a C, but the resulting codons (TAT and TAC) both code for tyrosine.

Source

The basic sequence comes from Ecogene.com

References