Difference between revisions of "Part:BBa K3038005:Design"
(→Source) |
(→Design Notes) |
||
| Line 10: | Line 10: | ||
A 6 his Tag has been added at the Cterm part of the TesA enzyme | A 6 his Tag has been added at the Cterm part of the TesA enzyme | ||
| − | A | + | A cMyc tag has been added at the Nterm part of the Mlut enzyme |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 13:23, 19 October 2019
PBAD/TesACTer6His/MlutNterCmyc
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
All the coding sequences have been designed with the RFC10 standard and optimized for the GC content.
A 6 his Tag has been added at the Cterm part of the TesA enzyme A cMyc tag has been added at the Nterm part of the Mlut enzyme
Source
Cloning of the different parts using the Biobrick design and classic cloning techniques
References
Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels Helge Jans Janßen1 and Alexander Steinbüchelcorresponding author1,2. Biotechnol Biofuels. 2014; 7: 7. doi: 10.1186/1754-6834-7-7. PMCID: PMC3896788. PMID: 24405789
Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80.doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610