Difference between revisions of "Part:BBa K3038005:Design"
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===References===
 
===References===
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Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels Helge Jans Janßen1 and Alexander Steinbüchelcorresponding author1,2. Biotechnol Biofuels. 2014; 7: 7. doi: 10.1186/1754-6834-7-7. PMCID: PMC3896788. PMID: 24405789
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Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80.doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610

Revision as of 13:22, 19 October 2019


PBAD/TesACTer6His/MlutNterCmyc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the coding sequences have been designed with the RFC10 standard and optimized for the GC content.

A 6 his Tag has been added at the Cterm part of the TesA enzyme A <cmyc tag has been added at the Nterm part of the Mlut enzyme


Source

Cloning of the different part using the Biobrick design and classic cloning techniques

References

Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels Helge Jans Janßen1 and Alexander Steinbüchelcorresponding author1,2. Biotechnol Biofuels. 2014; 7: 7. doi: 10.1186/1754-6834-7-7. PMCID: PMC3896788. PMID: 24405789


Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80.doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610