Difference between revisions of "Part:BBa K2922029"

 
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<partinfo>BBa_K2922029 short</partinfo>
 
<partinfo>BBa_K2922029 short</partinfo>
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===Summary===
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Lysis proteins are required for both colicin release and partial cell lysis.
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Abbreviated as Nkil, lysis protein for Colicin-N is a 5.6 kDa protein, which is encoded by gene <i>cnl</i>. The lysis protein is required for both Colicin-E1 release and partial cell lysis[1]. (Fig.1)
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<table><tr><th>[[File:Ndesign.png|thumb|300px|Fig.1 Nkil helps Colicin-N release and makes partial cell lysis.]]</th><th></table>
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===Identification===
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===Usage and Biology===
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When we are building this circuit, we are doing the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we send the plasmid to sequence, and get the correct sequencing.
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After new molecular cloning experiments, we do Enzyme-Cut identification to certify the plasmid is correct. We use the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we getting the target separate fragment-159bp. (Fig.2)
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<table><tr><th>[[File:Nkil-Ekil.png|thumb|300px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>
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<span class='h3bb'>Sequence and Features</span>
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===Reference===
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[1] A. P. Pugsley, The immunity and lysis genes of ColN plasmid pCHAP4. Molecular & General Genetics Mgg 211, 335-341 (1988).
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===Sequence and Features===
 
<partinfo>BBa_K2922029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2922029 SequenceAndFeatures</partinfo>
  

Revision as of 13:21, 19 October 2019


Lysis protein for colicin-N coding region

Summary

Abbreviated as Nkil, lysis protein for Colicin-N is a 5.6 kDa protein, which is encoded by gene cnl. The lysis protein is required for both Colicin-E1 release and partial cell lysis[1]. (Fig.1)

Fig.1 Nkil helps Colicin-N release and makes partial cell lysis.

Identification

When we are building this circuit, we are doing the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we send the plasmid to sequence, and get the correct sequencing. After new molecular cloning experiments, we do Enzyme-Cut identification to certify the plasmid is correct. We use the XbaI and PstI to cut the plasmid, then we getting the target separate fragment-159bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] A. P. Pugsley, The immunity and lysis genes of ColN plasmid pCHAP4. Molecular & General Genetics Mgg 211, 335-341 (1988).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 118
  • 1000
    COMPATIBLE WITH RFC[1000]