Difference between revisions of "Part:BBa K3038005:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
all the coding sequences have been designed with the RFC10 standard and optimized for the GC content.
+
All the coding sequences have been designed with the RFC10 standard and optimized for the GC content.
  
 
A 6 his Tag has been added at the Cterm part of the TesA enzyme  
 
A 6 his Tag has been added at the Cterm part of the TesA enzyme  
Line 16: Line 16:
 
===Source===
 
===Source===
  
−
cloning of the different part using the Biobrick design and classic cloning techniques  
+
Cloning of the different part using the Biobrick design and classic cloning techniques  
  
 
===References===
 
===References===

Revision as of 13:20, 19 October 2019


PBAD/TesACTer6His/MlutNterCmyc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the coding sequences have been designed with the RFC10 standard and optimized for the GC content.

A 6 his Tag has been added at the Cterm part of the TesA enzyme A <cmyc tag has been added at the Nterm part of the Mlut enzyme


Source

Cloning of the different part using the Biobrick design and classic cloning techniques

References