Difference between revisions of "Part:BBa K2951008"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This is a fusion protein of LysRS and Hemagglutinin. LysRS is lysyl tRNA synthase from E.coli str. K-12 substr. MG1655, it is found that it could act as a fusion protein partner and increase the solubility of the other significantly. Hemagglutinin is a surface glycoprotein of influenza viruses, and can be used in immunological experiments as the target antigen. Hemagglutinin is one of the transmembrane proteins that are considered difficult to express as soluble form in E.coli. Combining the two parts together, we call it as “LysRS-HA”. This is also an improvement of a previous part BBa_K1955000. In addition to expressing hemagglutinin in soluble form efficiently, we also added His-tag for further purification, TEV cutting site for removal of LysRS after expression, and a polylinker in order for others to construct a plasmid and applications with other hemagglutinin subtypes or even other proteins that are expressed as insoluble form in E.coli. | This is a fusion protein of LysRS and Hemagglutinin. LysRS is lysyl tRNA synthase from E.coli str. K-12 substr. MG1655, it is found that it could act as a fusion protein partner and increase the solubility of the other significantly. Hemagglutinin is a surface glycoprotein of influenza viruses, and can be used in immunological experiments as the target antigen. Hemagglutinin is one of the transmembrane proteins that are considered difficult to express as soluble form in E.coli. Combining the two parts together, we call it as “LysRS-HA”. This is also an improvement of a previous part BBa_K1955000. In addition to expressing hemagglutinin in soluble form efficiently, we also added His-tag for further purification, TEV cutting site for removal of LysRS after expression, and a polylinker in order for others to construct a plasmid and applications with other hemagglutinin subtypes or even other proteins that are expressed as insoluble form in E.coli. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 13:14, 19 October 2019
LysRS-Hemagglutinin fusion protein
LysRS-Hemagglutinin fusion protein
Contents
Usage and Biology
This is a fusion protein of LysRS and Hemagglutinin. LysRS is lysyl tRNA synthase from E.coli str. K-12 substr. MG1655, it is found that it could act as a fusion protein partner and increase the solubility of the other significantly. Hemagglutinin is a surface glycoprotein of influenza viruses, and can be used in immunological experiments as the target antigen. Hemagglutinin is one of the transmembrane proteins that are considered difficult to express as soluble form in E.coli. Combining the two parts together, we call it as “LysRS-HA”. This is also an improvement of a previous part BBa_K1955000. In addition to expressing hemagglutinin in soluble form efficiently, we also added His-tag for further purification, TEV cutting site for removal of LysRS after expression, and a polylinker in order for others to construct a plasmid and applications with other hemagglutinin subtypes or even other proteins that are expressed as insoluble form in E.coli.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 275
Illegal NotI site found at 1256 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 864
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1845
- 1000COMPATIBLE WITH RFC[1000]