Difference between revisions of "Part:BBa K2924043"

Revision as of 13:04, 19 October 2019

Hpall promoter

The P_HpaII promoter¹, is a strong constitutive promoter that is commonly used for expression in gram-positive bacteria

Usage and Biology

The promoter originated from the gram positive bacterium </i>Staphylococcus aureus</i> and has better affinity to the binding site of RNA polymerase or other transcription activators which help in increasing the transcription rate of downstream DNA to messenger RNA^2,3,4. P_HpaII promoter stimulates counterclockwise RNA synthesis⁵. Comparable expression strength to that of P₄₃ (BBa_K208002)². In our case it was used to produce high amounts of heterologous protein (BBa_K2924033, BBa_K2924052) that is secreted into the culture supernatant.

References

[1]: Westers, L., Dijkstra, D.S., Westers, H., van Diji, J.M., Quax, W.J. (2006). "Secretion of functional human interleukin-3 from Bacillus subtilis." Journal of biotechnology 123.2 (2006): 211-224.

[2]: Liu S. L., Du K. (2012). Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme. Protein Expr Purif. 2012 Jun;83(2):164-8. doi: 10.1016/j.pep.2012.03.015. Epub 2012 Apr 4

[3]: Guan C., Cui W., Cheng J., Zhou L., Guo J., Hu X., Xiao G., Zhou Z. (2015). Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015; 14: 150. doi: 10.1186/s12934-015-0341-2.

[4]: Chen, Jingqi, et al. "Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein." Biotechnology letters 37.4 (2015): 899-906.

[5]: Zhang, Kang, et al. "High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system." Microbial cell factories 16.1 (2017): 32.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2924043 short</partinfo>
-Hpall promoter
+The P<sub>HpaII</sub> promoter<sup>1</sup>, is a strong constitutive promoter that is commonly used for expression in gram-positive bacteria
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The promoter originated from the gram positive bacterium </i>Staphylococcus aureus</i> and has better affinity to the binding site of RNA polymerase or other transcription activators which help in increasing the transcription rate of downstream DNA to messenger RNA<sup>2,3,4</sup>. P<sub>HpaII</sub> promoter stimulates counterclockwise RNA synthesis<sup>5</sup>. Comparable expression strength to that of P<sub>43</sub> (BBa_K208002)<sup>2</sup>. In our case it was used to produce high amounts of heterologous protein (BBa_K2924033, BBa_K2924052) that is secreted into the culture supernatant.
+===References===
+[1]: Westers, L., Dijkstra, D.S., Westers, H., van Diji, J.M., Quax, W.J. (2006). "Secretion of functional human interleukin-3 from Bacillus subtilis." Journal of biotechnology 123.2 (2006): 211-224.
+[2]: Liu S. L., Du K. (2012). Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme. Protein Expr Purif. 2012 Jun;83(2):164-8. doi: 10.1016/j.pep.2012.03.015. Epub 2012 Apr 4
+[3]: Guan C., Cui W., Cheng J., Zhou L., Guo J., Hu X., Xiao G., Zhou Z. (2015). Construction and development of an auto-regulatory gene expression system in Bacillus subtilis. Microb Cell Fact. 2015; 14: 150. doi: 10.1186/s12934-015-0341-2.
+[4]: Chen, Jingqi, et al. "Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein." Biotechnology letters 37.4 (2015): 899-906.
+[5]: Zhang, Kang, et al. "High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system." Microbial cell factories 16.1 (2017): 32.
 <!-- -->