Difference between revisions of "Part:BBa K2922024"

 
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<partinfo>BBa_K2922024 short</partinfo>
 
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===Summary===
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This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
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As a kind of bacteriocin secreted by <i>E.coli</i>, colicin can kill other related bacteria that can’t secret specific immunity proteins.
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For Colicin-E1, it is a type of pore-forming colicin encoded by gene <i>cea</i>.This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.[1] (Fig.1)
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<table><tr><th>[[File:E1design.png|thumb|300px|Fig.1 Colicin-E1 can kill other bacteria.]]</th><th></table>
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===Usage and Biology===
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===Identification===
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When we are building this circuit, we are doing the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we send the plasmid to sequence, and get the correct sequencing.
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After new molecular cloning experiments, we do Enzyme-Cut identification to certify the plasmid is correct. We use the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we getting the target separate fragment-1560bp. (Fig.2)
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<span class='h3bb'>Sequence and Features</span>
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<table><tr><th>[[File:E1 N.png|thumb|300px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>
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===Reference===
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[1] E. Cascales et al., Colicin biology. Microbiol Mol Biol Rev 71, 158-229 (2007).
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===Sequence and Features===
 
<partinfo>BBa_K2922024 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2922024 SequenceAndFeatures</partinfo>
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Revision as of 13:03, 19 October 2019


Colicin-E1 coding region

Summary

As a kind of bacteriocin secreted by E.coli, colicin can kill other related bacteria that can’t secret specific immunity proteins. For Colicin-E1, it is a type of pore-forming colicin encoded by gene cea.This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.[1] (Fig.1)

Fig.1 Colicin-E1 can kill other bacteria.

Identification

When we are building this circuit, we are doing the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we send the plasmid to sequence, and get the correct sequencing. After new molecular cloning experiments, we do Enzyme-Cut identification to certify the plasmid is correct. We use the XbaI and PstI to cut the plasmid, then we getting the target separate fragment-1560bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] E. Cascales et al., Colicin biology. Microbiol Mol Biol Rev 71, 158-229 (2007).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1369
    Illegal AgeI site found at 1501
  • 1000
    COMPATIBLE WITH RFC[1000]