Difference between revisions of "Part:BBa K2963030"

 
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This promoter is a hybrid of two operons: the trp and lac operons. This promoter is inducible by IPTG and commonly used in Corynebacterium glutamicum for overproduction of proteins. Corynebacterium glutamicum constitutively produces the LacIq protein, a strong pTAC promoter repressor which can bind with lac operator. In our experiment, we add two lac operators in order to forbid leaked expression of downstream genes.
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This promoter is a hybrid of two operons: the trp and lac operons. This promoter is inducible by IPTG and commonly used in <i>Corynebacterium glutamicum</i> for overproduction of proteins. <i>Corynebacterium glutamicum</i> constitutively produces the LacI protein, a strong Ptac promoter repressor which can bind with lac operator. In our experiment, we add two lac operators in order to regulate the expression of the <i>racE<i/> gene to produce γ-PGA with different D/L-glutamate monomer ratios.
  
 
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Revision as of 12:55, 19 October 2019


Tac Promoter (including two operator genes(lacO))


This promoter is a hybrid of two operons: the trp and lac operons. This promoter is inducible by IPTG and commonly used in Corynebacterium glutamicum for overproduction of proteins. Corynebacterium glutamicum constitutively produces the LacI protein, a strong Ptac promoter repressor which can bind with lac operator. In our experiment, we add two lac operators in order to regulate the expression of the racE<i/> gene to produce γ-PGA with different D/L-glutamate monomer ratios.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]