Difference between revisions of "Part:BBa K2973002"
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As expected, the non-regulated eGFP emitted the highest fluorescence, providing a positive control and confirming the protocol we used was functional. The toehold-regulated construct produced robust signal in decreasing concentrations of trigger (75nM, 15nM, 7nM). It is interesting that the signal remained the same even with a 10-fold decrease in trigger concentration, while the no trigger control had an almost 2-fold lower fluorescence. | As expected, the non-regulated eGFP emitted the highest fluorescence, providing a positive control and confirming the protocol we used was functional. The toehold-regulated construct produced robust signal in decreasing concentrations of trigger (75nM, 15nM, 7nM). It is interesting that the signal remained the same even with a 10-fold decrease in trigger concentration, while the no trigger control had an almost 2-fold lower fluorescence. | ||
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Revision as of 12:39, 19 October 2019
T7-RBS- enhanced green fluorescent protein (EGFP)
This composite part consists of T7 Promoter (BBa_J64997) and T7 Terminator(BBa_K731721), Ribosomal Binding Site(BBa_B0034) and EGFP CDS (BBa_K1094400). EGFP (Enhanced Green Fluorescent Protein) is a version of GFP(green fluorescent protein) that produces enhanced bioluminescence signal in the green zone of the noticeable spectrum and is being used widely as a protein reporter. This protein is produced by the jellyfish (Aequorea Victoria).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 776
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]