Difference between revisions of "Part:BBa K3182009"

Revision as of 12:29, 19 October 2019

Parseq-α

This part is a de novo antimicrobial peptide. The antimicrobial peptide was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea do to there hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptide in different concentration. they were then incubated at 37°C with shaking every 15 minutes. the plate was incubated for 16 hours and OD⁶⁰⁰ was measured. The results from this can be seen in Figure 1.. Parseq alpha showed an inhibiting effect on the growth of E.coli and B.subtilis.

Figure 1 Shows the antimicrobial activity of Parseq Alpha on A) E.Coli. and B) B.subtilis. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C. shaking was conducted for 1 minute every 15 minutes and the OD⁶⁰⁰ were messured every hour for 16 hours. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad

Figure 1. Shows the antimicrobial activity of Parseq Alpha on B.Subtilis. The OD⁶⁰⁰ value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 [[File:T--Linkoping_Sweden--AlphaEcoli.png|650px|thumb|center|<b>Figure 1</b> Shows the antimicrobial activity of Parseq Alpha
-  on <i>E.Coli.</i>. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. NG stands for no growth and MIC stands for minimum inhibitory concentration. The positive control was E.coli cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD. ]]
+  on A) <i>E.Coli.</i> and B) <i>B.subtilis</i>. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C. shaking was conducted for 1 minute every 15 minutes and the OD<sup>600</sup> were messured every hour for 16 hours. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad ]]
 [[File:T--Linkoping Sweden--alphaBsubtilis.png|650px|thumb|center|<b>Figure 1.</b> Shows the antimicrobial activity of Parseq Alpha on B.Subtilis. The OD<sup>600</sup> value is displayed on the y-axis and the concentrations tested can be seen in on the x-axis. The positive control was B.Subtilis cultured without antimicrobial peptides. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C for 16 hours. All measurements are done in 6 replicates. Error bars represent SD.]]