Difference between revisions of "Part:BBa K3278000"
| Line 30: | Line 30: | ||
<img src="https://2019.igem.org/wiki/images/thumb/0/04/T--ShanghaiTech_China--characterization_18%28pme_36h%29.png/800px-T--ShanghaiTech_China--characterization_18%28pme_36h%29.png" alt="pme_36h" width="790" height="635"></p> | <img src="https://2019.igem.org/wiki/images/thumb/0/04/T--ShanghaiTech_China--characterization_18%28pme_36h%29.png/800px-T--ShanghaiTech_China--characterization_18%28pme_36h%29.png" alt="pme_36h" width="790" height="635"></p> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| + | <p> | ||
| + | To see more changes on pDawn, go to the websites below.</p> | ||
| + | <p> | ||
| + | pDawn+mEGFP: BBa_K3278000</p> | ||
| + | <p> | ||
| + | pDawn with T7 promoter: BBa_3278001</p> | ||
| + | <p> | ||
| + | pDawn with mtac promoter: BBa_K3278002 (good)</p> | ||
| + | <p> | ||
| + | pdawn with tac promoter: BBa_K3278005</p> | ||
| + | <p> | ||
| + | pDawn with YF2: BBa_K3278006 (good)</p> | ||
| + | <html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 12:27, 19 October 2019
pDawn-mEGFP
This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.
0 hour:
6 hour:
12 hour:
24 hour:
30 hour:
36 hour:
To see more changes on pDawn, go to the websites below.
pDawn+mEGFP: BBa_K3278000
pDawn with T7 promoter: BBa_3278001
pDawn with mtac promoter: BBa_K3278002 (good)
pdawn with tac promoter: BBa_K3278005
pDawn with YF2: BBa_K3278006 (good)
Sequence and Features