Difference between revisions of "Part:BBa K3278000"

Line 33: Line 33:
  
 
To see more changes on pDawn, go to the websites below.
 
To see more changes on pDawn, go to the websites below.
pDawn+mEGFP BBa_K3278000
+
 
https://parts.igem.org/Part:BBa_K3278000
+
pDawn+mEGFP: BBa_K3278000
pDawn with T7 promoter BBa_3278001
+
 
https://parts.igem.org/Part:BBa_K3278001
+
pDawn with T7 promoter: BBa_3278001
pDawn with mtac promoter BBa_K3278002
+
 
https://parts.igem.org/Part:BBa_K3278002
+
pDawn with mtac promoter: BBa_K3278002 (good)
pdawn with tac promoter BBa_K3278005
+
 
https://parts.igem.org/Part:BBa_K3278005
+
pdawn with tac promoter: BBa_K3278005
pDawn with YF2 BBa_K3278006
+
 
https://parts.igem.org/Part:BBa_K3278006
+
pDawn with YF2: BBa_K3278006 (good)
pDawn+NAS  BBa_K3278007
+
 
  
  

Revision as of 12:26, 19 October 2019


pDawn-mEGFP

This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.

0 hour: pme_0h

6 hour: pme_6h

12 hour: pme_12h

24 hour: pme_24h

30 hour: pme_30h

36 hour: pme_36h

To see more changes on pDawn, go to the websites below. pDawn+mEGFP: BBa_K3278000 pDawn with T7 promoter: BBa_3278001 pDawn with mtac promoter: BBa_K3278002 (good) pdawn with tac promoter: BBa_K3278005 pDawn with YF2: BBa_K3278006 (good) Sequence and Features BBa_K3278000 SequenceAndFeatures