Difference between revisions of "Part:BBa K145001"
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This RNA polymerase will start transcription from a T7 promoter (such as [https://parts.igem.org/Part:BBa_I712074 BBa_I712074]).
 
This RNA polymerase will start transcription from a T7 promoter (such as [https://parts.igem.org/Part:BBa_I712074 BBa_I712074]).
  
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===Usage and Biology===
 
===Usage and Biology===
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<h2> <b> BOKU-Vienna 2019 - Improvement </b> </h2>
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Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, the Team BOKU-Vienna decided to remove those recognition sites by codon swapping to improve the applicability, you can find the functioning Polymerase under [[Part:BBa_K3015006|BBa_K3015006]]
  
 
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Revision as of 12:26, 19 October 2019

T7 RNA polymerase

This RNA polymerase will start transcription from a T7 promoter (such as BBa_I712074).

Usage and Biology

BOKU-Vienna 2019 - Improvement

Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, the Team BOKU-Vienna decided to remove those recognition sites by codon swapping to improve the applicability, you can find the functioning Polymerase under BBa_K3015006

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]