Difference between revisions of "Part:BBa K3278005"
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<partinfo>BBa_K3278005 short</partinfo> | <partinfo>BBa_K3278005 short</partinfo> | ||
| − | + | pDawn is a blue light control system which can start the expression of target gene under the inducement of blue light. More information in BBa_K1075044 added by iGEM13_Bonn. | |
| + | However, expression leakage and delay are the two main problems of pdawn system. Leakage refers to the expression of target protein without light. According to our measurement, samples strictly kept away from light still had significant expression of GFP after being cultured for one night. Therefore, we carefully studied the gene circuits of pdawn system [2] [3] and hypothesized that enhancing the strength of the promoter of YF1 may enhance the repression of protein expression in the dark, thus solving the leakage problem. | ||
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| + | In this part, we use the tac promoter to substitute the lacIq promoter in front of YF1 and Fixk2, trying to see whether it reduce the leakage and maintain good amount of expression. | ||
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| + | <html> | ||
| + | <p> | ||
| + | <b><font size="3"> 0 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/c/cb/T--ShanghaiTech_China--characterization_19%28tac_0h%29.png/447px-T--ShanghaiTech_China--characterization_19%28tac_0h%29.png" alt="tac_0h" width="395" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 6 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/3/35/T--ShanghaiTech_China--characterization_20%28tac_6h%29.png/800px-T--ShanghaiTech_China--characterization_20%28tac_6h%29.png" alt="tac_6h" width="790" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 12 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/a/a3/T--ShanghaiTech_China--characterization_21%28tac_12h%29.png/800px-T--ShanghaiTech_China--characterization_21%28tac_12h%29.png" alt="tac_12h" width="790" height="635"></p> | ||
| + | |||
| + | <p> | ||
| + | <b><font size="3"> 24 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/3/32/T--ShanghaiTech_China--characterization_22%28tac_24h%29.png/800px-T--ShanghaiTech_China--characterization_22%28tac_24h%29.png" alt="tac_24h" width="790" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 30 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/4/4f/T--ShanghaiTech_China--characterization_23%28tac_30h%29.png/800px-T--ShanghaiTech_China--characterization_23%28tac_30h%29.png" alt="tac_30h" width="790" height="635"></p> | ||
| + | |||
| + | <p> | ||
| + | <b><font size="3"> 36 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/d/d9/T--ShanghaiTech_China--characterization_24%28tac_36h%29.png/800px-T--ShanghaiTech_China--characterization_24%28tac_36h%29.png" alt="tac_36h" width="790" height="635"></p> | ||
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| + | <html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:22, 19 October 2019
pDawn with tac promoter
pDawn is a blue light control system which can start the expression of target gene under the inducement of blue light. More information in BBa_K1075044 added by iGEM13_Bonn.
However, expression leakage and delay are the two main problems of pdawn system. Leakage refers to the expression of target protein without light. According to our measurement, samples strictly kept away from light still had significant expression of GFP after being cultured for one night. Therefore, we carefully studied the gene circuits of pdawn system [2] [3] and hypothesized that enhancing the strength of the promoter of YF1 may enhance the repression of protein expression in the dark, thus solving the leakage problem.
In this part, we use the tac promoter to substitute the lacIq promoter in front of YF1 and Fixk2, trying to see whether it reduce the leakage and maintain good amount of expression.
0 hour:
6 hour:
12 hour:
24 hour:
30 hour:
36 hour:
