Difference between revisions of "Part:BBa K3278000"
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This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation. | This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation. | ||
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| + | <b><font size="3"> 0 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/1/1d/T--ShanghaiTech_China--characterization_13%28pme_0h%29.png/447px-T--ShanghaiTech_China--characterization_13%28pme_0h%29.png" alt="pme_0h" width="395" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 6 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/4/4d/T--ShanghaiTech_China--characterization_14%28pme_6h%29.png/800px-T--ShanghaiTech_China--characterization_14%28pme_6h%29.png" alt="pme_6h" width="790" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 12 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/4/42/T--ShanghaiTech_China--characterization_15%28pme_12h%29.png/800px-T--ShanghaiTech_China--characterization_15%28pme_12h%29.png" alt="pme_12h" width="790" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 24 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/9/99/T--ShanghaiTech_China--characterization_16%28pme_24h%29.png/800px-T--ShanghaiTech_China--characterization_16%28pme_24h%29.png" alt="pme_24h" width="790" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 30 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/f/fc/T--ShanghaiTech_China--characterization_17%28pme_30h%29.png/800px-T--ShanghaiTech_China--characterization_17%28pme_30h%29.png" alt="pme_30h" width="790" height="635"></p> | ||
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| + | <p> | ||
| + | <b><font size="3"> 36 hour:</font></b> | ||
| + | <img src="https://2019.igem.org/wiki/images/thumb/0/04/T--ShanghaiTech_China--characterization_18%28pme_36h%29.png/800px-T--ShanghaiTech_China--characterization_18%28pme_36h%29.png" alt="pme_36h" width="790" height="635"></p> | ||
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Revision as of 12:06, 19 October 2019
pDawn-mEGFP
This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.
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36 hour:
