Difference between revisions of "Part:BBa K3278002"
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In this part, we use the modified tac promoter(abbreviated as mtac promoter) to substitute the lacIq promoter in front of YF1 and Fixk2, largely reducing the leakage while maintain reasonable expression under blue light. | In this part, we use the modified tac promoter(abbreviated as mtac promoter) to substitute the lacIq promoter in front of YF1 and Fixk2, largely reducing the leakage while maintain reasonable expression under blue light. | ||
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| + | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:30, 19 October 2019
pDawn with modified tac promoter
pDawn is a blue light control system which can start the expression of target gene under the inducement of blue light. More information in BBa_K1075044 added by iGEM13_Bonn.
However, expression leakage and delay are the two main problems of pdawn system. Leakage refers to the expression of target protein without light. According to our measurement, samples strictly kept away from light still had significant expression of GFP after being cultured for one night. Therefore, we carefully studied the gene circuits of pdawn system [2] [3] and hypothesized that enhancing the strength of the promoter of YF1 may enhance the repression of protein expression in the dark, thus solving the leakage problem.
In this part, we use the modified tac promoter(abbreviated as mtac promoter) to substitute the lacIq promoter in front of YF1 and Fixk2, largely reducing the leakage while maintain reasonable expression under blue light.
0 hour:
6 hour:
