Difference between revisions of "Part:BBa K2924035:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence is codon-optimized for Synechocystis sp. PCC 6803. It was provided by Anna Behle. | + | The sequence is codon-optimized for Synechocystis sp. PCC 6803. It was provided by Anna Behle<sup>1</sup>. |
===References=== | ===References=== | ||
Revision as of 11:07, 19 October 2019
mVenus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 630
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
Illegal AgeI site found at 151 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is codon-optimized for Synechocystis sp. PCC 6803. It was provided by Anna Behle1.
References
Kremers, Gert-Jan, et al. "Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius." Biochemistry 45.21 (2006): 6570-6580.
Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.