Difference between revisions of "Part:BBa K3081006"
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<partinfo>BBa_K3081006 short</partinfo>
 
<partinfo>BBa_K3081006 short</partinfo>
  
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which a polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:54, 19 October 2019


pBAD-dCas9-J23119-polyA

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, in which polyA sequence is the control sgRNA targeted no binding site of E.coli genome replication initiation region, OriC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961