Difference between revisions of "Part:BBa K2951006"
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[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2444022/ 1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)] | [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2444022/ 1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)] | ||
===<span class='h3bb'>Sequence and Features</span>=== | ===<span class='h3bb'>Sequence and Features</span>=== | ||
| − | <partinfo> | + | <partinfo>BBa_K2951006 SequenceAndFeatures</partinfo> |
Latest revision as of 08:53, 19 October 2019
LysRS fusion protein
Usage and Biology
This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI(NC000913.3). LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.
Characterization
This part is part of the composite part: BBa_K2951008 and further characterized.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 275
Illegal NotI site found at 1256 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 864
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]