Difference between revisions of "Part:BBa K3038003"
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Thioesterase III (FadM) is a long-chain acyl-CoA thioesterase that is involved in the β-oxidation of oleic acid. The enzyme is able to hydrolyze a number of related substrates. The best substrate is 3,5-tetradecadienoyl-CoA, which is a minor side product of oleate β-oxidation that is resistant to further degradation. The hydrolysis product, 3,5-tetradecadienoate, is released into the growth medium [Ren04a, Nie08]. Thioesterase III is expressed upon growth on oleic acid as the sole source of carbon [Ren04a, Nie08]. FadM is a member of the fad regulon; expression is induced by a number of fatty acids, with C18:1 as the best inducer [Feng09b]. Reports disagree on whether [Nie08a] or not [Feng09b] conjugated linoleic acid (CLA) induces an even higher level of expression of fadM. | Thioesterase III (FadM) is a long-chain acyl-CoA thioesterase that is involved in the β-oxidation of oleic acid. The enzyme is able to hydrolyze a number of related substrates. The best substrate is 3,5-tetradecadienoyl-CoA, which is a minor side product of oleate β-oxidation that is resistant to further degradation. The hydrolysis product, 3,5-tetradecadienoate, is released into the growth medium [Ren04a, Nie08]. Thioesterase III is expressed upon growth on oleic acid as the sole source of carbon [Ren04a, Nie08]. FadM is a member of the fad regulon; expression is induced by a number of fatty acids, with C18:1 as the best inducer [Feng09b]. Reports disagree on whether [Nie08a] or not [Feng09b] conjugated linoleic acid (CLA) induces an even higher level of expression of fadM. | ||
| − | https://biocyc.org/gene?orgid=ECOLI&id=G6244 | + | https://biocyc.org/gene?orgid=ECOLI&id=G6244<br/> |
| − | + | For the competition, FadM is tagged in Cterm by a FLAG tag. This tag allows the purification of the protein in order to test the activity. | |
===GenBank=== | ===GenBank=== | ||
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===Protein Sequence === | ===Protein Sequence === | ||
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MQTQIKVRGY HLDVYQHVNN ARYLEFLEEA RWDGLENSDS FQWMTAHNIA | MQTQIKVRGY HLDVYQHVNN ARYLEFLEEA RWDGLENSDS FQWMTAHNIA | ||
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FVVVNININY RRPAVLSDLL TITSQLQQLN GKSGILSQVI TLEPEGQVVA | FVVVNININY RRPAVLSDLL TITSQLQQLN GKSGILSQVI TLEPEGQVVA | ||
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DALITFVCID LKTQKALALE GELREKLEQM VK | DALITFVCID LKTQKALALE GELREKLEQM VK | ||
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Molecular size : 15.088 kD (from nucleotide sequence) | Molecular size : 15.088 kD (from nucleotide sequence) | ||
| − | + | ==Reference== | |
Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610 | Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610 | ||
| − | + | ==Usage and Biology== | |
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Revision as of 08:22, 19 October 2019
Description
FadM is for E. coli long-chain acyl CoA thioesterase that is Thioesterase III.
Thioesterase III (FadM) is a long-chain acyl-CoA thioesterase that is involved in the β-oxidation of oleic acid. The enzyme is able to hydrolyze a number of related substrates. The best substrate is 3,5-tetradecadienoyl-CoA, which is a minor side product of oleate β-oxidation that is resistant to further degradation. The hydrolysis product, 3,5-tetradecadienoate, is released into the growth medium [Ren04a, Nie08]. Thioesterase III is expressed upon growth on oleic acid as the sole source of carbon [Ren04a, Nie08]. FadM is a member of the fad regulon; expression is induced by a number of fatty acids, with C18:1 as the best inducer [Feng09b]. Reports disagree on whether [Nie08a] or not [Feng09b] conjugated linoleic acid (CLA) induces an even higher level of expression of fadM.
https://biocyc.org/gene?orgid=ECOLI&id=G6244
For the competition, FadM is tagged in Cterm by a FLAG tag. This tag allows the purification of the protein in order to test the activity.
GenBank
FadM : GenBank: P77712
https://www.uniprot.org/uniprot/P77712
Protein Sequence
Without the Flag-tag :
MQTQIKVRGY HLDVYQHVNN ARYLEFLEEA RWDGLENSDS FQWMTAHNIA
FVVVNININY RRPAVLSDLL TITSQLQQLN GKSGILSQVI TLEPEGQVVA
DALITFVCID LKTQKALALE GELREKLEQM VK
Molecular size : 15.088 kD (from nucleotide sequence)
Reference
Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]