Difference between revisions of "Part:BBa K3071010"

 
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<partinfo>BBa_K3071010 short</partinfo>
 
<partinfo>BBa_K3071010 short</partinfo>
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===Description===
  
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===Biology===
 
RNA polymerase Sigma-54 factor is the initiation factor that promotes the attachment of RNA polymerase to the specific initiation site and its release. Naturally, this sigma factor is responsible for the expression of enzymes involved in arginine catabolism.  
 
RNA polymerase Sigma-54 factor is the initiation factor that promotes the attachment of RNA polymerase to the specific initiation site and its release. Naturally, this sigma factor is responsible for the expression of enzymes involved in arginine catabolism.  
  
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The activation of sigma54-promotor requires the participation of an activator that binds to upstream elements and the contact between the activator and the sig54-RNAP holoenzyme. The contact point for transcription regulator, promote DNA strand separation, influencing the early phase of elongation, and promoter-proximal pausing. Transcription activation require ATP hydrolysis and leads to a conformational change to an open complex for transcription.
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===Usage===
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<center>[[file:T--Hong_Kong-CUHK--sigma_54_activation.png]]</center>
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<center<u>Figure 1 The transcription initiation mechanism of the sigma-54 promotors</u></center> 
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The activation of the sigma54-promotor requires the participation of an activator that binds to upstream elements and the contact between the activator and the sigma 54-RNAP holoenzyme. The contact point for transcription regulator, promote DNA strand separation, influencing the early phase of elongation, and promoter-proximal pausing. Transcription activation requires ATP hydrolysis and leads to a conformational change to an open complex for transcription.
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Revision as of 07:35, 19 October 2019


RNA polymerase Sigma-54 factor (rpoN)

Description

Biology

RNA polymerase Sigma-54 factor is the initiation factor that promotes the attachment of RNA polymerase to the specific initiation site and its release. Naturally, this sigma factor is responsible for the expression of enzymes involved in arginine catabolism.

Usage

T--Hong Kong-CUHK--sigma 54 activation.png

<centerFigure 1 The transcription initiation mechanism of the sigma-54 promotors</center> The activation of the sigma54-promotor requires the participation of an activator that binds to upstream elements and the contact between the activator and the sigma 54-RNAP holoenzyme. The contact point for transcription regulator, promote DNA strand separation, influencing the early phase of elongation, and promoter-proximal pausing. Transcription activation requires ATP hydrolysis and leads to a conformational change to an open complex for transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 796
  • 1000
    COMPATIBLE WITH RFC[1000]