Difference between revisions of "Part:BBa K3202059"

 
Line 3: Line 3:
 
<partinfo>BBa_K3202059 short</partinfo>
 
<partinfo>BBa_K3202059 short</partinfo>
  
−
Arac-pC-pBAD-lacI-B0034-PhiC31-T500-T1/TE-MicC sRNA-sRNA Binding site-PA1/04
+
Alternative version of BBa_K3202048, sfGFP is replaced by recombinase PhiC31
  
 +
In former studies, we find out that AraC-Pc-pBAD-LacI system can provide the most rigorous results. Therefore, we created the first bistable system in our design by repLacIng sfGFP with recombinases.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:44, 19 October 2019


Xyls-Pc-Pm-TetR-RBS1-PhiC31-T500-T1/TE-MicCsRNA-sRNA Binding site-PLtetO

Alternative version of BBa_K3202048, sfGFP is replaced by recombinase PhiC31

In former studies, we find out that AraC-Pc-pBAD-LacI system can provide the most rigorous results. Therefore, we created the first bistable system in our design by repLacIng sfGFP with recombinases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 999
    Illegal NheI site found at 1022
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 930
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1775
    Illegal SapI site found at 2118
    Illegal SapI site found at 2223
    Illegal SapI site found at 2281
    Illegal SapI.rc site found at 1972