Difference between revisions of "Part:BBa K2967025:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | They come from the registry. | |
===References=== | ===References=== | ||
| + | Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016). | ||
Latest revision as of 06:41, 19 October 2019
The yeaR promoter added an extra NsrR Binding Sequences
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs.
Source
They come from the registry.
References
Merulla, D. & van der Meer, J. R. Regulatable and modulable background expression control in prokaryotic synthetic circuits by auxiliary repressor binding sites. ACS Synth. Biol. 5, 36–45 (2016).