Difference between revisions of "Part:BBa K3254014"
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<partinfo>BBa_K3254014 short</partinfo> | <partinfo>BBa_K3254014 short</partinfo> | ||
| + | This part is a twin of [[Part:BBa_K2572025|BBa_K2572025]], we creat this page just for more detailed sequence annotations. | ||
This promoter was optimized from Ptac promoter([[Part:BBa_K864400|BBa_K864400]]) through using a symmetrical lacO site. After combined with the lacI constitutive expression cassette([[Part:BBa_K3254022|BBa_K3254022]]), it can be regulated by the IPTG inducer. A mutant library with different dynamic range had been built(see [[Part:BBa_K3254015|BBa_K3254015]],[[Part:BBa_K3254016|BBa_K3254016]] and [[Part:BBa_K3254017|BBa_K3254017]]). | This promoter was optimized from Ptac promoter([[Part:BBa_K864400|BBa_K864400]]) through using a symmetrical lacO site. After combined with the lacI constitutive expression cassette([[Part:BBa_K3254022|BBa_K3254022]]), it can be regulated by the IPTG inducer. A mutant library with different dynamic range had been built(see [[Part:BBa_K3254015|BBa_K3254015]],[[Part:BBa_K3254016|BBa_K3254016]] and [[Part:BBa_K3254017|BBa_K3254017]]). | ||
Revision as of 04:56, 19 October 2019
Optimized Ptac promoter
This part is a twin of BBa_K2572025, we creat this page just for more detailed sequence annotations. This promoter was optimized from Ptac promoter(BBa_K864400) through using a symmetrical lacO site. After combined with the lacI constitutive expression cassette(BBa_K3254022), it can be regulated by the IPTG inducer. A mutant library with different dynamic range had been built(see BBa_K3254015,BBa_K3254016 and BBa_K3254017).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]