<b>This part (BBa_K3132016) is a MAPK pathway-dependent synthetic receptor that targets SunTag peptide.</b>
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GEMS, which is also named as Generalized Extracellular Molecule Sensor, is a highly generalizable modular platform that was first published in 2018. In this literature, the author constructed sensors for non-signaling molecules with a wide range of molecular weights to confirm the validity and generalizability of the GEMS, and found these GEMS devices are the first examples of 2.sensing soluble molecules with scFv-coupled receptors that 1.utilize natural signaling cascades rewired for transgene expression,unlike the previous membrane surface receptors which can only detect membrane marker. 3.The easy adaptability of the GEMS platform to new targets should make it a useful tool for many applications in synthetic biology and for developing novel precision-guided cell-based therapeutics.
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<B>Structure of GEMS:</B>
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GEMS consists of three structural domains: an extracellular domain, a transmembrane domain, and an intracellular domain (fig.1).The extracellular domain is based on an EpoR scaffold. After introducing a mutation into the erythropoietin receptor to render it inert to erythropoietin, it can be fused to affinity domains such as antibody fragments, then the two form a whole as extracellular receptor for signals. The EpoR transmembrane domain was fused to the intracellular signal transduction domains. Once activated, these intracellular domains can induce downstream signaling pathways and regulate gene expression.
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<B>Mechanism of GEMS:</B>
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Inactive EpoR dimers are locked by transmembrane helix interactions in a conformation that prevents downstream signaling. Ligand binding to the receptors is thought to rotate each receptor subunit around its own axis and is likely accompanied by an increase in the distance between intracellular domains. The combination of these effects triggers downstream signaling (fig.2).
This part (BBa_K3132016) is a MAPK pathway-dependent synthetic receptor that targets SunTag peptide.
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1477
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2163 Illegal BglII site found at 2444 Illegal BamHI site found at 70 Illegal BamHI site found at 1770 Illegal XhoI site found at 64 Illegal XhoI site found at 991 Illegal XhoI site found at 2303
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2348
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2234 Illegal BsaI.rc site found at 2598 Illegal SapI.rc site found at 1566 Illegal SapI.rc site found at 1581
