Difference between revisions of "Part:BBa K577881"

 
Line 17: Line 17:
 
<partinfo>BBa_K577881 parameters</partinfo>
 
<partinfo>BBa_K577881 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<h1> XHD-WS-Wuhan-B 2019's Characterization</h1>
 +
Here, we used K577881 part, an inducible araC-pBAD promoter with GFP , to investiagte effect on different concentrations of arabniose on GFP expression in this part, and to provide a more detailed data for this part.
 +
1. Growth curve of E.coli containing part K577881 in different concentration of L-arabinose
 +
As shown in Fig.1, the growth of bacteria was not influenced at the range of 500 -10000μM of L-arabinose. When the concentration of L-arabinose reached 50000mΜ, a little inhibition was observed, indicating a little toxicity to cells at this level. 
 +
Fig.1

Revision as of 02:30, 19 October 2019


pC+AraC+pBad+GFPc

This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a GFP gene. The whole part is flanked by the regular E, X and S, P restriction sites, so it can be approached with the usual restriction digest method. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the GFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1923
    Illegal SapI site found at 961


XHD-WS-Wuhan-B 2019's Characterization

Here, we used K577881 part, an inducible araC-pBAD promoter with GFP , to investiagte effect on different concentrations of arabniose on GFP expression in this part, and to provide a more detailed data for this part. 1. Growth curve of E.coli containing part K577881 in different concentration of L-arabinose As shown in Fig.1, the growth of bacteria was not influenced at the range of 500 -10000μM of L-arabinose. When the concentration of L-arabinose reached 50000mΜ, a little inhibition was observed, indicating a little toxicity to cells at this level. Fig.1