Difference between revisions of "Part:BBa K2936001"

Revision as of 01:20, 19 October 2019

pBAD-LacZɑ

The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction.

The LacZ alpha gene is one of the structural genes present on the Lac Operon in E. coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene encoding β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) will be expressed.

Usage and Biology

Figure 1 The A350 of reaction solution of bacterial culture and TMB
Figure 2 The mechanism of the color reaction
From figure 1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD6000=0.5) to TMB is designed to be 1:10 .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 120
Illegal BamHI site found at 182
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-Form 1 The A350 of reaction solution of bacterial culture and TMB
+Figure 1 The A350 of reaction solution of bacterial culture and TMB
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    <img src="https://static.igem.org/mediawiki/parts/4/47/T--ZJUT-China--igem-part01yanse.jpg" alt=""width="600px">
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-Figure 1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right
+Figure 2 The mechanism of the color reaction
 <br>
 From figure 1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD6000=0.5) to TMB is designed to be 1:10 .