Difference between revisions of "Part:BBa K3114010"
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===Usage and Biology===
 
===Usage and Biology===
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BBa_K3114010 is a golden gate compatible part which allows insertion of the SGR gene into genetic circuits to express the enzyme magnesium dechelatase.
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The conversion of chlorophyll a to pheophytin a is the first step in the natural chlorophyll degradation pathway. The enzyme Magnesium Dechelatase, encoded by the SGR gene, catalyzes this reaction by extracting magnesium (Mg<sup>2+</sup>) from chlorophyll, along with cofactors 2H<sup>+</sup> (Shimoda et al. 2016).
  
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==Collection of Parts==
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BBa_K3114010 is a golden gate compatible part which allows insertion of the SGR gene into genetic circuits to express the enzyme Magnesium Dechelatase. In creating this part, iGEM Calgary wished to allow the golden gate cloning of this plant enzyme into <i>E.coli</i> for other teams to use in projects involving degradation of chlorophyll. This part was designed to be golden-gate compatible based on the Mo-Clo standard (Weber et al., 2011). It is also compatible with the BioBrick RFC[10] standard.
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This part was designed to be Golden Gate compatible with certain Golden Gate parts in iGEM Calgary's 2019 part collection including the following: <br/>
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1. DsbA signal peptide - Bba_K3114000 https://parts.igem.org/Part:BBa_K3114000<br/>
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This part is compatible with other golden gate parts created by iGEM Calgary including the following:
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2. MalE signal peptide - Bba_K3114001 https://parts.igem.org/Part:BBa_K3114001<br/>
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<ul>
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3. OmpA signal peptide - Bba_K3114002 https://parts.igem.org/Part:BBa_K3114002<br/>
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        <li>DsbA signal peptide [https://parts.igem.org/Part:BBa_K3114000 (BBa_K3114000)]</li>
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4. PhoA signal peptide - Bba_K3114003 https://parts.igem.org/Part:BBa_K3114003<br/>
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<li>MalE signal peptide [https://parts.igem.org/Part:BBa_K3114001 (BBa_K3114001)]</li>  
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5. Tat signal peptide - Bba_K3114004 https://parts.igem.org/Part:BBa_K3114004<br/>
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<li>OmpA signal peptide [https://parts.igem.org/Part:BBa_K3114002 (BBa_K3114002)]</li>  
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6. TorA signal peptide - Bba_K3114005 https://parts.igem.org/Part:BBa_K3114005<br/>
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<li>PhoA signal peptide [https://parts.igem.org/Part:BBa_K3114003 (BBa_K3114003)]</li>
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7. Double Terminator  - Bba_K3114013 https://parts.igem.org/Part:BBa_K3114013
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<li>TorA signal peptide [https://parts.igem.org/Part:BBa_K3114005 (BBa_K3114005)]</li>
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<li>YcbK signal peptide [https://parts.igem.org/Part:BBa_K3114004 (BBa_K3114004)]</li>
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        <li>Double Terminator  [https://parts.igem.org/Part:BBa_K3114013 (BBa_K3114013)]</li>
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</ul>
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===Sequences and Features===
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<partinfo>BBa_K3114010 SequenceAndFeatures</partinfo>
  
 
===References===
 
===References===
 
Shimoda, Y., Ito, H., & Tanaka, A. (2016). Arabidopsis STAY-GREEN, Mendel’s green cotyledon gene, encodes magnesium-dechelatase. The Plant Cell, 28(9), 2147-2160.
 
Shimoda, Y., Ito, H., & Tanaka, A. (2016). Arabidopsis STAY-GREEN, Mendel’s green cotyledon gene, encodes magnesium-dechelatase. The Plant Cell, 28(9), 2147-2160.
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3114010 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 23:40, 18 October 2019


Magnesium Dechelatase Stay Green (SGR)

The conversion of chlorophyll a to pheophytin a is the first step in the natural chlorophyll degradation pathway. The enzyme Magnesium Dechelatase, encoded by the SGR gene, catalyzes this reaction by extracting magnesium (Mg2+) from chlorophyll, along with cofactors 2H+ (Shimoda et al. 2016)

Usage and Biology

The conversion of chlorophyll a to pheophytin a is the first step in the natural chlorophyll degradation pathway. The enzyme Magnesium Dechelatase, encoded by the SGR gene, catalyzes this reaction by extracting magnesium (Mg2+) from chlorophyll, along with cofactors 2H+ (Shimoda et al. 2016).

BBa_K3114010 is a golden gate compatible part which allows insertion of the SGR gene into genetic circuits to express the enzyme Magnesium Dechelatase. In creating this part, iGEM Calgary wished to allow the golden gate cloning of this plant enzyme into E.coli for other teams to use in projects involving degradation of chlorophyll. This part was designed to be golden-gate compatible based on the Mo-Clo standard (Weber et al., 2011). It is also compatible with the BioBrick RFC[10] standard.

This part is compatible with other golden gate parts created by iGEM Calgary including the following:


Sequences and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1508
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1883

References

Shimoda, Y., Ito, H., & Tanaka, A. (2016). Arabidopsis STAY-GREEN, Mendel’s green cotyledon gene, encodes magnesium-dechelatase. The Plant Cell, 28(9), 2147-2160.