Difference between revisions of "Part:BBa K137076"

Revision as of 17:24, 17 October 2008

AHL inducible production of hydrogen peroxide

This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.

Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H₂O₂ production assay], K137076 in pSB1A2 in JI377 produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for JI377 with an empty plasmid).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2741
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1005

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H<sub>2</sub>O<sub>2</sub> production assay], K137076 in pSB1A2 in DH10B produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for DH10B with an empty plasmid).
+Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H<sub>2</sub>O<sub>2</sub> production assay], K137076 in pSB1A2 in JI377 produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for JI377 with an empty plasmid).
 [[Image:K137076.gif]]