Difference between revisions of "Part:BBa K3165008:Design"
 
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<partinfo>BBa_K3165009 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3165008 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 21:12, 18 October 2019

N-Terminal T7 RNAP Domain + nMag (Optimised for B. subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 678
    Illegal BsaI site found at 1573
    Illegal BsaI.rc site found at 2107
    Illegal BsaI.rc site found at 2146


Design Notes

To generate the two separate domains of the T7 RNA Polymerase, the protein was cleaved at the 563rd - 564th amino acid and linked to the light-sensitive heterodimerizing units via GGSGG linker sequences. This part was codon optimised for Bacillus subtilis.


Source

The sequence of this part was derived from the supplementary material of "Dynamic blue light-inducible T7 RNA polymerases (Opto-T7RNAPs) for precise spatiotemporal gene expression control". Department of Biosystems Science and Engineering (D-BSSE), ETH–Zürich.

References