Difference between revisions of "Part:BBa K3071020"

 
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<partinfo>BBa_K3071020 short</partinfo>
 
<partinfo>BBa_K3071020 short</partinfo>
  
This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp (BBa_K3071009) with the Ribosome binding site (BBa_J61102) and C-terminus Myc-tag with a double stop codon (BBa_K3071017)
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This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp) ([https://parts.igem.org/Part:BBa_K3071009 BBa_K3071009]) with the Ribosome binding site ([https://parts.igem.org/Part:BBa_J61102 BBa_J61102]) and C-terminus Myc-tag with a double stop codon ([https://parts.igem.org/Part:BBa_K3071017 BBa_K3071017])
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:00, 18 October 2019


Xcc Clp-pspF fused with Myc-tag with RBS

This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp) (BBa_K3071009) with the Ribosome binding site (BBa_J61102) and C-terminus Myc-tag with a double stop codon (BBa_K3071017)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 925
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 928
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 925
    Illegal NgoMIV site found at 1003
    Illegal AgeI site found at 108
    Illegal AgeI site found at 136
    Illegal AgeI site found at 274
  • 1000
    COMPATIBLE WITH RFC[1000]