Difference between revisions of "Part:BBa K3071020"
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<partinfo>BBa_K3071020 short</partinfo> | <partinfo>BBa_K3071020 short</partinfo> | ||
− | This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp (BBa_K3071009) with the Ribosome binding site (BBa_J61102) and C-terminus Myc-tag with a double stop codon (BBa_K3071017) | + | This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp) ([https://parts.igem.org/Part:BBa_K3071009 BBa_K3071009]) with the Ribosome binding site ([https://parts.igem.org/Part:BBa_J61102 BBa_J61102]) and C-terminus Myc-tag with a double stop codon ([https://parts.igem.org/Part:BBa_K3071017 BBa_K3071017]) |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 21:00, 18 October 2019
Xcc Clp-pspF fused with Myc-tag with RBS
This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp) (BBa_K3071009) with the Ribosome binding site (BBa_J61102) and C-terminus Myc-tag with a double stop codon (BBa_K3071017)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 925
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 928
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 925
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 925
Illegal NgoMIV site found at 1003
Illegal AgeI site found at 108
Illegal AgeI site found at 136
Illegal AgeI site found at 274 - 1000COMPATIBLE WITH RFC[1000]