Difference between revisions of "Part:BBa K2984016"
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created. | This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created. | ||
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| + | This part was designed to be used with the MoClo standard and has B5-B5 overhangs. | ||
Revision as of 20:34, 18 October 2019
SP20 B5-B5 - Glycomodule for enhancement of secretion in C. reinhardtii
This part is a part of the Chlamy-HUB-Collection. It has previously been shown that the fusion of a glycomodule at the C-terminal end of a protein enhances the secretion up to 12-fold and also increases the proteolytic protein stability (Ramos-Martinez et al., 2017). A tandem serine and proline of 20 repeats (SP20) module was therefore created.
This part was designed to be used with the MoClo standard and has B5-B5 overhangs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Ramos‐Martinez, E. M., Fimognari, L., & Sakuragi, Y. (2017). High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii. Plant biotechnology journal, 15(9), 1214-1224.