Difference between revisions of "Part:BBa K2984014"

Revision as of 20:33, 18 October 2019

6x His-Tag for protein detection, isolation, purification B5-B5

This part is a part of the Chlamy-HUB-Collection. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2⁺ or Co2⁺ making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2<sup>+</sup> or Co2<sup>+</sup> making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.
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+This part was designed to be used with the MoClo standard and has B5-B5 overhangs.
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