Difference between revisions of "Part:BBa K3110054"
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<partinfo>BBa_K3110054 short</partinfo> | <partinfo>BBa_K3110054 short</partinfo> | ||
| − | + | YebF is a 13kDa protein that is known to be secreted into the extracellular medium by E. coli strains used in the lab. This particular protein was used with IL-12 in our design, in order to mediate the secretion of the cytokine from the bacteria. The part was used with constitutive promoter (J23100) but the results were inconclusive. Western blots were done with both the cell pellet and concentrated supernatant but the protein was not detected.Possible errors could be because of SOEing errors in the promoter and RBS regions. | |
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| + | The original YebF part can be found here: BBa_K1659003 | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 20:25, 18 October 2019
J23117-YebF IL12-FLAG
YebF is a 13kDa protein that is known to be secreted into the extracellular medium by E. coli strains used in the lab. This particular protein was used with IL-12 in our design, in order to mediate the secretion of the cytokine from the bacteria. The part was used with constitutive promoter (J23100) but the results were inconclusive. Western blots were done with both the cell pellet and concentrated supernatant but the protein was not detected.Possible errors could be because of SOEing errors in the promoter and RBS regions.
The original YebF part can be found here: BBa_K1659003
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]