Difference between revisions of "Part:BBa K2951006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI[https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3033657&to=3035174&strand=true NC000913.3]. LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD. | + | This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI[https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3033657&to=3035174&strand=true (NC000913.3)]. LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD. |
===Characterization=== | ===Characterization=== |
Revision as of 20:04, 18 October 2019
LysRS fusion protein
Usage and Biology
This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI(NC000913.3). LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.
Characterization
This part is part of the composite part: BBa_K2951008 and further characterized.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 291
- 1000COMPATIBLE WITH RFC[1000]