Difference between revisions of "Part:BBa K2951006"
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===Usage and Biology===
 
===Usage and Biology===
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This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBI[https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3033657&to=3035174&strand=true NC000913.3]. LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.
  
 
===Characterization===
 
===Characterization===

Revision as of 20:03, 18 October 2019

LysRS fusion protein

Usage and Biology

This part contains the original sequence of lysyl tRNA synthase (LysRS/LysS) from E.coli str. K-12 substr. MG1655. The sequence is obtained from NCBINC000913.3. LysRS is a homodimeric protein (114 kDa), and its monomer consists of N-terminal (LysN) and C-terminal catalytic domains. The LysN domain binds to the anticodon of tRNA-Lys and was expected to serve as an independent RBD.

Characterization

This part is part of the composite part: BBa_K2951008 and further characterized.

References

1.Seong Il Choi, Kyoung Sim Han, Chul Woo Kim, Ki-Sun Ryu, Byung Hee Kim, Kyun-Hwan Kim, Seo-Il Kim, Tae Hyun Kang, Hang-Cheol Shin, Keo-Heun Lim, Hyo Kyung Kim, Jeong-Min Hyun, and Baik L. Seong, Protein Solubility and Folding Enhancement by Interaction with RNA, PLoS ONE 3(7)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]