Difference between revisions of "Part:BBa K3110047"

Revision as of 19:29, 18 October 2019

Promoter RBS FimH 136KO-RPMrel

FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to RPMrel. We amplified the FimHRPMrel from this existing part, and to this, we ligated a strong constitutive promoter and strong RBS for greater expression. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 549
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3110047 short</partinfo>
-FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the ------ position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to  RPMrel. We amplified the FimHRPMrel from this existing part, and to this, we ligated a strong constitutive promoter and strong RBS for greater expression.
+FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to  RPMrel. We amplified the FimHRPMrel from this existing part, and to this, we ligated a strong constitutive promoter and strong RBS for greater expression.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===