Difference between revisions of "Part:BBa K3110034"

Revision as of 19:15, 18 October 2019

Medium Promoter Strong RBS lldP+lldR+lldD

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 328
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3650
Illegal AgeI site found at 464
Illegal AgeI site found at 927
Illegal AgeI site found at 1365
Illegal AgeI site found at 1524
Illegal AgeI site found at 1536
Illegal AgeI site found at 2282
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3087

Revision as of 20:12, 14 October 2019 (view source) Registry (Talk \| contribs)		Revision as of 19:15, 18 October 2019 (view source) Meghamariajacob (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3110034 short</partinfo>		<partinfo>BBa_K3110034 short</partinfo>

−	~~L-Lactate Dehydrogenase (lldD) and~~ L-Lactate Permease (lldP) ~~and the regulator~~ element (lldR) under the control of a medium promoter and a strong RBS.	+	This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.