Difference between revisions of "Part:BBa K3187015"
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| − | BBa_K1073024 encodes the yellow chromoprotein amilGFP derived from the coral <i>Acropora millepora</i><sup id="cite_ref-5" class="reference"> | + | BBa_K3187015 (improved BBa_K1073024) version of encodes the yellow chromoprotein amilGFP derived from the coral <i>Acropora millepora</i><sup id="cite_ref-5" class="reference"> |
<a href="#cite_note-5">[5] </a> </sup> under the control of a strong constitutive promoter (<a | <a href="#cite_note-5">[5] </a> </sup> under the control of a strong constitutive promoter (<a | ||
href=" https://parts.igem.org/Part:BBa_J23100" | href=" https://parts.igem.org/Part:BBa_J23100" | ||
Revision as of 19:14, 18 October 2019
Constitutively expressed chromoprotein amilGFP with Translation Enhancing 5'-UTR
Improved Version of BBa_K1073024
BBa_K3187015 (improved BBa_K1073024) version of encodes the yellow chromoprotein amilGFP derived from the coral Acropora millepora [5] under the control of a strong constitutive promoter (BBa_J23100) in combination with a ribosomal binding site ( BBa_B0032). The improved and the original version of the part were cloned into the pSB1C3 backbone and transformed in E. coli BL21 (DE3).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]