Difference between revisions of "Part:BBa K3110030"

Revision as of 19:03, 18 October 2019

Weak Promoter Weak RBS lldP+lldD

This construct has L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a weak RBS. lldD cleaves L-Lactate to pyruvate and lldP permits the entry of L-Lactate inside the bacterial cell. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldP by varying the strength of the promoter and RBS controlling their production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 327
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2857
Illegal AgeI site found at 463
Illegal AgeI site found at 926
Illegal AgeI site found at 1364
Illegal AgeI site found at 1523
Illegal AgeI site found at 1535
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2294

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3110030 short</partinfo>
-L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a weak RBS.
+This construct has L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a weak RBS. lldD cleaves L-Lactate to pyruvate and lldP permits the entry of L-Lactate inside the bacterial cell. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldP by varying  the strength of the promoter and RBS controlling their production.
 <!-- Add more about the biology of this part here