Difference between revisions of "Part:BBa K3110019"

 
Line 3: Line 3:
 
<partinfo>BBa_K3110019 short</partinfo>
 
<partinfo>BBa_K3110019 short</partinfo>
  
−
L-Lactate Dehydrogenase (lldD) and Regulator element (lldR) under the control of a weak promoter and a strong RBS.
+
This construct has L-Lactate Dehydrogenase (lldD) and Regulator element (lldR) under the control of a weak promoter and a strong RBS. lldD cleaves L-Lactate to pyruvate and lldR has the dual role of an activator and a repressor.  Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various  higher and lower concentrations of lldD and lldR by varying  the strength of the promoter and RBS controlling their production.
 +
 
  
  

Revision as of 18:31, 18 October 2019


Weak Promoter Strong RBS lldR+lldD

This construct has L-Lactate Dehydrogenase (lldD) and Regulator element (lldR) under the control of a weak promoter and a strong RBS. lldD cleaves L-Lactate to pyruvate and lldR has the dual role of an activator and a repressor. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldR by varying the strength of the promoter and RBS controlling their production.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1988
    Illegal AgeI site found at 620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1425